Acrp30 (adipocyte complement-related protein of 30 kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28 kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q.
Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation.
Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance.
Acrp30 (mouse 247 aa, rat human 244 aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14 aa), followed by a 27-aa unique region, and then by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus.
Acrp30 is proteolytically cleaved at 104 aa to generate the globular Acrp30 (gAcrp30).
Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-5ug/ml (ECL)
ELISA: 1:5000-25,000.
Coat plate with 10-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: A0725-03
Molecular Weights:Full length Acrp30: ~30kD
Recombinant Acrp30: ~37kDgAcrp30:~16kD.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes mouse Acrp30. Detects both the globular (truncated) and full length Acrp30 proteins because the epitope is located near the C-terminus. No significant sequence similarity with the related protein C1q. Species Sequence Homology: Bovine Acrp30: 100%; human and monkey Acrp30: 90%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic 11aa peptide sequence near the C-terminus of mouse gAcrp30 (KLH coupled). Epitope: C terminus
