84-1039-92　［取扱停止］Acid Sensitive Ion Channel 3 (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) 100ul　A0576-05

特徴

• Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain.
• Proton-gated cation channels are activated by low pH in nociceptive neurons.
• H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC), (3) and mechanosensory channel proteins of nematode degenerins (DEG).
• NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop.
• All members of this family are selective for Na+ and blocked by amiloride.
• The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASIC for Acid Sensing Ion Channels).
• Three are at least three distinct proteins in ASIC family: ASIC1 (identical with human BNAC2 or BNC2), expressed in brain and dorsal root ganglions (DRG) cells, is activated by pH <7.0.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL
• ELISA: 1:10,000-1:50,000.
• Coat ELISA plates at 1ug/ml with Control Peptide
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:A0576-06: Acid Sensitive Ion Channel 3, Rat, CT, 21aa (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) Control Peptide.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.