In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA.
In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step.
The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation.
Activities of ACC-1 and -2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK).
Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism.
ACC-2 (rat 2456aa, human 2483aa, ~280kD, chromosome 12q24.1), also known as ACC-beta, is predominantly present in heart and skeletal muscle and to a lesser extent in liver.
An additional ACC-2 isoform (270kD) is present in liver.
In contrast to ACC-1, which is cytosolic and catalyzes only fatty acid synthesis, ACC-2 co-localizes with CPT-1 in the 'contact sites' of the mitochondrial membranes and regulates mitochondrial fatty acid oxidation as well by inhibiting CPT-1 by its product malonyl-CoA.
ACC-2 contains an unique 114aa long N-terminus peptide, accounting in part, for its regulatory role in fatty acid oxidation.
ACC2 deficient mice accumulate 10-30 fold less malonyl-CoA in heart and muscle and show 50% less fat in the adipose tissue.
Applications:Suitable for use in ELISA.
Although not tested, Western Blot may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng of A0225-03B per wellOptimal dilutions to be determined by the researcher.
Recommended Control Peptide:A0225-03B: AcCoA Carboxylase 2, Control Peptide, Rat
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes rat AcCoA Carboxylase 2. No significant sequence homology is seen with ACC-1 or any other protein.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide consisting of 20aa sequence near the N-terminus of rat ACC-2, conjugated to KLH. Species Sequence Homology: mouse, 75%; human, 60%.
