Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease.
DNase I is synthesized in the pancreas and stored in zymogen granules.
It has been used to reduce the viscosity of cystic fibrosis sputum.
A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis.
A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000 Western Blot: 1:500-1:2000; D3200-05B can be used as a positive control
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes bovine Deoxyribonuclease I. Expected to crossreact with other mammalian DNases from human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo
Immunogen：Purified bovine pancreatic DNase I (EC 3.1.21.1).
