Paxillin is a multi-domain protein that localizes primarily in focal adhesion sites to the extracellular matrix (1).
Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2).
Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at tyrosine 118 (3,4).
Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions.
It has been shown that theSH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).
Applications:Suitable for use in Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Protein A and peptide affinity chromatography
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol
Specificity：Detects endogenous levels of paxillin from various cell lines. Does not crossreact with other related proteins
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (KLH coupled) corresponding to residues near the carboxy- terminal sequence of human paxillin
