The oxidation of L-arginine by a family of nitric oxide synthase (NOS) isoenzymes, nNOS, eNOS, and iNOS, is responsible for the production of nitric oxide (NO).
In mammalian genomes, three separate genes encode the NOS isoforms, two expressed constitutively (nNOS, eNOS), and one inducible (iNOS).
Endothelial NOS (eNOS, NOS III) is expressed in endothelial cells, some neurons and cardiac myocytes, and is responsible for cardiovascular homeostatic processes, including blood pressure regulation, vessel remodeling and angiogenesis (1)
eNOS undergoes a unique translocation cycle between the plasma membrane and the cytoplasm, suggesting that its intracellular localization is important in linking stimuli to eNOS enzymatic activity.
Phosphorylation of the protein at several sites also plays a regulatory role.
Phosphorylation of eNOS at Ser1179 in response to stimulation by VEGF has been observed to enhance eNOS activity, increasing the production of NO (1), while phosphorylation at Thr497 in combination with dephosphorylation at Ser1179 inhibits eNOS activity (2).
Ser1179 (bovine)/Ser1177 (human) is the target residue for phosphorylation of multiple protein kinases, including Akt (3), AMP kinase (4), PKA and protein kinase G (5), and CaM kinase II (6).
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/mlImmunofluorescence: Reactive with monkey COS cells (1) Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes bovine eNOS protein, phosphorylated at Ser1179 (~135kD). Reactivity has been confirmed with VEGF-stimulated, serum-starved BAEC (bovine aortic endothelial cell) lysates. A very low signal was observed in the lysates of serum-starved, unstimulated BAEC and no signal was observed in lysates of unstimulated BAEC in the presence of serum. Species Sequence Homology: human, rat, mouse, canine, porcine and guinea pig
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Bo
Immunogen：Synthetic peptide corresponding to the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.
