84-1039-39　Claudin 7 (CLDN7) 100ug　C5838-55

特徴

• Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets.
• In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell.
• TJ strands on adjacent cells are presumed to interact with each other to form a sort of “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other.
• In addition to this so-called “barrier” function, the “fence” function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces.
• Claudin-7 is localized in the intercellular junctions of the epithelial cells of the proximal tubules of the nephrons in mammalian kidney1.
• It is reportedly down regulated in head and neck squamous cell carcinomas2.
• Claudin-7 is also expressed in normal human and mouse epithelia.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence (Canine): 1-2ug/ml Western Blot: 1-2ug/ml Immunohistochemistry (Human, mouse): 1-2ug/ml.
• Prior to staining, formalin-fixed, paraffin-embedded tissues requires heat-induced epitope retrieval (HIER) with citrate buffer, pH 6.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.