Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets.
TJ strands on adjacent cells are presumed to interact with each other to form a “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other.
The 20 known claudin proteins are major structural components of tight junctions.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1.0ug/ml Western Blot: 0.5-3ug/ml Immunohistochemistry: 0.1-1.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes mouse Claudin-3 at 22kD.Species Crossreactivity: Mouse liver and lung tissue extract and canine MDCK and human MCF-7 cell lysates by Western Blot and formalin-fixed, paraffin-embeddedd human normal colon and colon cancer tissues by Immunohistochemistry.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：A synthetic peptide from the C-terminus of mouse Claudin-3
