STATs were originally identified as DNA-binding proteins essential to interferon IFNa-and IFNg-regulated gene expression.
Since then, several additional mammalian STATs, a Drosophila STAT (D-STAT or Marelle), and a STAT-like protein from Dictyostelium discoideum have been recognized.
In unstimulated cells STAT proteins exist largely as latent cytoplasmic transcription factors.
Treatment of cells with cytokines or certain growth factors results in the tyrosine phosphorylation (via Janus kinases for cytokines) of specific STATs causing them to form homodimers or heterodimers that enter the nucleus.
In the nucleus, STAT dimers bind DNA and activate transcription of distinct target genes.
Although several different STATs may be activated in response to a given ligand, there is often a striking specificity in the pattern of STAT activation.
Mammalian STAT proteins share several conserved structural and functional domains as illustrated below.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot (Human, mouse and rat): 1-3ug/mlELISA (Immunogen): 0.1-1ug/ml Immunohistochemistry (Mouse): 5-10ug/ml.
Parafin tissues requires HIER with citrate buffer pH 6.0
Optimal dilutions to be determined by the researcher.
Controls:STAT5 transfected 293 cells expressing either wild type or inactive JAK1 kinase, A431and K562 cell lysates prepared before and after treatment with epidermal growth factor.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes the tyrosine phosphorylated form of mouse STAT5a and STAT5b proteins. It does not crossreact with the corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine containing proteins. Species Crossreactivity: human and rat. Species Sequence Homology: bovine and sheep
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic tyrosine phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y694) of mouse STAT5.
