Vascular endothelial growth inhibitor (VEGI) belongs to the human tumor necrosis factor (TNF) family and acts as a multifunctional cytokine.
VEGI is expressed predominantly in endothelial cells and is an endothelial cell-specific negative regulator of angiogenesis.
Human VEGI protein contains 174 amino acids.
The calculated molecular weight is approximately 20kD.
An experiment examining in vitro transcription and translation involving the use of a cDNA clone as a template revealed a similar molecular weight of approximately 22kD.
Hydrophobicity analysis predicts a 13 amino acid hydrophobic region that follows the C-terminal segment of 12 residues; this is typical of a type II transmembrane protein with the C-terminus on the extracellular surface, a single transmembrane domain, and a short cytoplasmic tail.
Northern blots reveal that VEGI transcripts have been observed in the endothelial cells of placenta, lung, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine, and colon.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human VEGI at~20kD. Reacts with human PC-3 prostate adenocarcinoma cell lysates.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the C-terminal sequence of human VEGI.
