84-1038-93　Annexin I 100ul　A2295-16

特徴

• The Annexins are a structurally related family of calcium-binding proteins that associate reversibly with membranes, and bind to phospholipid bilayers in the presence of micromolar free calcium concentrations.
• This binding is specific for calcium and for acidic phospholipids.
• Each of the annexin proteins consist of an N-terminal domain of variable length followed by four or eight copies of a conserved segment of sixty one residues.
• The repeat (sometimes known as an ‘endonexin fold’) consists of five alpha-helices that are wound into a right-handed superhelix.
• Annexins have been demonstrated to be involved in cytoskeletal interactions, phospholipase inhibition, intracellular signaling, anticoagulation, and membrane fusion.
• Although the annexins are primarily intracellular proteins and lack hydrophobic signal sequences, some family members (e.g.
• Annexin I and V) have been found to be secreted.
• Annexin I (calpactin II, chromobindin 9, GIF, Lipocortin, p35) is a cytoskeletal calcium-dependent phospholipid-binding protein which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and it may be involved in secretion.
• Although this protein has received considerable attention due to its potential involvement in a variety of pathways, research progress on the localization of Annexin I during development and in pathophysiological conditions has been hampered due to conflicting reports on the immunolocalization of the protein.
• These contradictory data are believed to result in large part from unusual properties of the protein and its apparent sensitivity to different fixation conditions.
• Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence/Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000Immunofluorescence (IC): 1ug/mlImmunohistochemistry (Paraffin): 1:20-1:200Flow Cytometry: 3-5ug/million cellsOptimal dilutions to be determined by the researcher.
• Positive Control Lysates:Human fibroblasts, mouse kidney, mouse lung, adult rat and mouse brain
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.