14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins.
14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis including Raf, PKC, Bad, Cbl, and c-Bcr.
Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
14-3-3 expression is particularly high in certain neural tissues and studies in Drosophila have established a role for 14-3-3 proteins in nervous system development and function.
Recently, a dimeric 14-3-3 protein was determined to be an essential cofactor for Raf kinase activity.
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1.0ug/mlWestern Blot: 2ug/ml Immunofluorescence (IC): 2ug/ml See reference #1Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human and is broadly reactive with members of the 14-3-3 family of proteins (~29–32kD). Species Crossreactivity: bovine, C. Elegans, mouse, rat and Xenopus.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Bo Ce Hu Mo Rt Xe
Immunogen：Synthetic peptide corresponding to 20aa from the N-terminus of the human 14-3-3 beta/alpha protein. Sequence Homology: delta and zeta isoforms: single aa change; tau isoform: three aa change; gamma isoform: four aa change.
