Chloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+K+Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+Cl- cotransporters (KCC1-4).
These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites.
NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells.
The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.
Unlike NKCC1, NKCC2 (human 1099 aa, rat/mouse 1095 aa; calculated mol size of ~121kD; actual size ~150kD; ~45% identity with NKCC1) is most strongly expressed in the kidney (inner and outer stripes of medulla and cortical thick ascending limb), and macula densa.
NKCC2 plays a critical role in transcellular absorption of Na+Cl- by the medullary and cortical TALH, and a secondary role in the paracellular transport of Na-Ca and Mg.
Mutations in the NKCC2 gene result in Bartter's syndrome, an inherited disease characterized by hypokalemic metabolic alkalosis, hypercalciuria, salt wasting, and volume depletion.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
Full length huma NKCC2 is ~150kD.
ELISA: 1:10,000-1:50,000.
Using 1ug/ml control peptide per well.
Optimal dilutions to be determined by the researcher.
Control Peptide:N2801A: NKCC2, Control Peptide (Sodium, Potassium, Chloride Cotransporter 2, BSC1, CCC2)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat NKCC2, BSC1 and CCC2. The immunogen shows no significant sequence homology with NKCC1 or TSC.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 15 amino acid peptide sequence near the C-terminus, cytoplasmic domain of rat NKCC2 (KLH). Species sequence homology: mouse-100%, human and rabbit- 87%.
