Chloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells - the others are mostly impermeable organic anions.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+Cl- cotransporters (KCC1-4).
These co-transporters share a common predicted membrane topology, with 12 TM domains (~500aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites.
NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells.
The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.
NKCC1/BSC2 calculated mol.
wt of ~115kD; actual size is ~145kD.
It is strongly expressed in the kidney and many other tissues.
In mouse NKCC1 is detected in basolateral membrane of IMCD and in mesangial cells in the glomerulus and basolateral membrane of acinar cells in rat submandubular gland.
In the brain, NKCC1 mRNA is most prominent in choroid plexus.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ug/ml using ECL
ELISA: 1:10,000-1:50,000 using 1ug/ml N2800A control peptide per well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes rat sodium, potassium, chloride cotransporter 1 (NKCC1, BSC2, CCC1). The immunogen shows no significant sequence homology with other CCC.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide of 22aa near the C-terminus of rat NKCC1. (KLH). Cellular Localization: Cytoplasmic. Species sequence homology: mouse-95%, human-86% and bovine-82%.
