Type I plasminogen activator inhibitor, PAI 1, is a 50kD single- chain glycoprotein which belongs to the serine protease inhibitor (SERPIN) family of proteins.
The concentration of PAI 1 in normal human plasma is very low, with an average concentration of ~20ng/ml.
The plasma concentrations of PAI 1 can be affected by a number of factors including diurnal variations, age, sex, pregnancy, obesity and exercise status.
PAI 1 is synthesized by various cell types including endothelial cells, hepatocytes, vascular smooth muscle cells, fibroblasts, mesothelial cells, granulosa cells and malignant cell lines.
It is also found in the alpha-granules of platelets as well as plasma.
PAI 1 exists in at least three different conformations, including an active form with a half-life of ~1-3 hours, a latent form and a proteolytically or oxidatively inactivated form.
The plasma glycoprotein vitronectin has been shown to bind active PAI 1 and stabilize it in its active conformation, extending its functional half-life to greater than 24 hours.
Little specific binding of the latent or inactive forms of PAI 1 to vitronectin occurs.
PAI 1 is considered to be the primary regulator of plasminogen activation in vivo.
It is the major physiologic inhibitor of both the single chain and two-chain forms of tPA, being able to inhibit the single-chain form at least 10000 times greater than other PAIs.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl, A403/A280):0.41
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in buffered stabilizer solution, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Specificity：Recognizes human Plasminogen Activator Inhibitor Type 1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant Plasminogen Activator Inhibitor Type 1 (PAI 1) from bacterial extracts.
