Stat3 protein is a transcription factor expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1.
Stat3 binds to DNA as either a homodimer or as a heterodimer with Stat1.
Stat3 interacts with APRE (acute phase response element) and SIE (c-sis inducible element).
Applications:Suitable for use in Gel Supershift and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 5-10ug/ml for 2hrs at RT
Optimal dilution determined by the researcher.
Positive Control:Jurkat cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See S7971-01F
Specificity：Recognizes human STAT 3 (Signal Transducers and Activators of Transcription 3). Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide from C-terminus of human Stat3. MW of Antigen: 89kD. Epitope: C-terminal. Cellular Localization: Nuclear
