84-1038-81　［取扱停止］ROC1 500ul　R2997-80

特徴

• ROC1 and ROC2 are the two highly conserved RING finger proteins that are homologous to APC11, a subunit of the anaphase-promoting complex (APC).
• ROC1 and ROC2 commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-related APC subunit.
• ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1-and E2-dependent manner.
• ROC1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin1 to catalyze ubiquitin polymerization.
• Epitope:aa97-108
• Applications:Suitable for use in Immunoprecipitation and Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Immunoprecipitation: 10ug/mg protein lysate (Use Protein A; Native verified)Western Blot: 5-10ug/ml for 2hrs at RT
• Optimal dilutions to be determined by the researcher.
• Positive Control:HeLa cells and tonsil.
• Cellular Localization:Nuclear
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.