The Ki67 is a nuclear protein, which is expressed in the proliferating cells.
Ki67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiscent) phase are negative for this protein.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin/parafin): 10-20ug/ml for 30 min.
at RT.
Requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min
Optimal dilution determined by the researcher.
Positive Control:Tonsil or breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form： Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See K1700-13.
Specificity：Recognizes human Ki67. Excellent for staining of formalin/paraffin tissues and serves as a marker of growth fraction. Species Crossreactivity: Rat.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide from the human Ki67 protein. Cellular Localization: Nuclear Epitope: Middle of Ki67 protein.
