Factor XIII is a beta-globulin found in plasma and is composed of two subunits.
Factor XIIIa is the catalytic subunit and is a dimer of MW 160kD.
Factor XIII is present in plasma as an a2b2 heterodimer (M.W.
320kD); whereas in platelets, only the a2 unit exists.
Factor XIIIa is a dermal dendrocyte marker and shows variable reaction with these types of tumors.
It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system.
Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 5-10ug/ml for 2 hours at RTImmunohistochemistry (Formalin/paraffin): 10-20ug/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control:Human recombinant Factor XIIIa.
Capillary hemangioma, dermatofibroma, and placenta.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human Factor XIIIa at ~160kD (non-reduced dimer), ~80kD (reduced monomer). Does not crossreact with human Factor XIII B-chain or human Factor XII.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to A subunit of coagulation Factor XIII. Cellular Localization: Cytoplasmic and nuclear.
