ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition.
The ER is an important regulator of growth and differentiation in the mammary gland.
Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g.
tamoxifen) therapy.
Applications:Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation (denatured verified): 10ug/mg protein lysate.
Use Protein A
Western Blot: 2.5-5ug/ml for 2hrs at RT
Immunohistochemistry (formalin/paraffin): 10-20ug/ml for 30 min at RT.
Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.
Positive Control:MCF-7 cells.
Breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3565-06FX.
Specificity：Recognizes human Estrogen Receptor (ER) at 67kD. Species Crossreactivity: bovine, mouse, porcine, rabbit, rat and sheep
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Hu Mo Po Rb Rt Sh
Immunogen：Synthetic peptide from the N-terminus of human estrogen receptor, alpha protein. Cellular Localization: Nuclear.
