The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions.
b-catenin also binds to N-cadherin and coimmunoprecipitates with APC.
Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin.
Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
Applications:Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunoprecipitation (Native verified): 10ug/mg Use Protein A
Western Blot: 5-10ug/ml for 2 hours at RTImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control:MCF-7 cells.
Breast carcinoma.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2069-50BX.
Specificity：Recognizes human Catenin, beta at ~92kD. Species Crossreactivity: mouse, rat, chicken and Xenopus
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Ch Hu Mo Rt Xe
Immunogen：Synthetic peptide from the C-terminus of the Beta-catenin protein. Cellular Localization: Cytoplasmic and cell membrane
