Applications:Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Neutralization of Human IFN-g R1 Bioactivity:The exact concentration of antibody required to neutralize rhIFN-g R1-mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied.
The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response
The ND50 is ~0.5-2ug/ml in the presence of 2ng/ml of rhIFN-g, using a confluent culture of HeLa cells.
ELISA: 0.5-1ug/ml.
Direct ELISA.
The detection limit for rhIFN-g R1 is ~0.3ng/well
Western Blot: 0.1-0.2ug/ml.
The detection limit for is ~5ng/lane under non-reducing and reducing conditions
Immunohistochemistry: 15ug/ml.
Detects IFN-g R1 in human PBMC (peripheral blood mononuclear cells)’s (Fluorescence immunocytochemistry
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-buffered saline pH 7.2 (20mM Tris base,150mM sodium chloride), 0.1% BSA. No preservative added. Labeled with Biotin.
Specificity：Binds to and neutralizes human Interferon gamma receptor 1, 15.756kD. Blocks cell surface human human Interferon gamma receptor 1-mediated bioactivity of recombinant human Interferon gamma receptor 1. In direct ELISA and Western Blot, does not crossreact with human Interferon gamma receptor chain2.
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC IP N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant, human Interferon gamma receptor chain 1, NS0-derived, extracellular domain
