The calcitonin family peptides probably act through G-protein coupled membrane receptors.
The gene for calcitonin receptors has been cloned.
It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP).
Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified.
CRLR has 55% homology with calcitonin receptor.
It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.
RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus.
RAMP1-3 displays 31% identity.
RAMPS may be involved in the transport of CRLR to the plasma membrane.
RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor.
RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract.
CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors.
RAMP2 (human 175 aa; rat 182 aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor.
It is expressed in the lung, breast, immune system and fetal tissues.
RAMP3 is most abundant in the kidney and lung.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml.
Western Blot: 1-10ug/ml
Optimal dilutions to be determined by the researcher
Control Peptide: R1060-05A
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human RAMP1. Species sequence homology: mouse-52%, rat-91%. No significant sequence homology is detected with other RAMPs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 19aa and 11aa synthetic peptides mapped to the C-terminus and middle portion of human RAMP1, coupled to KLH.
