NFk B comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death.
In the cytoplasm NFk B is negatively modulated by the inhibitory proteins Ik B.
In turn Ik B is phosphorylated by a cellular kinase complex called IKK.
IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate Ik B leading to its degradation and the resulting translocation of NFk B to the nucleus.
IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF-a , endotoxins and overexpression of cellular kinases like MEKK1.
Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.
Applications:Suitable for use in Immunoprecipitation, Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Reactive against IKKa followed by reaction with I1904-46Q.
Detects a band at ~ 85kD.
Optimal dilutions to be determined by the researcher
ELISA: 1:5000-1:25,000Optimal dilutions to be determined by the researcher.
Control peptide: I3000-04B
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.01% sodium azide.
Specificity：Recognizes human total IKK alpha protein at 85kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：IKKa peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to KLH.
