The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses.
In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB.
An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB.
In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control.
Additionally, NFkB is thought to be an important transcriptional regulator for HIV.
Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.
Applications:Suitable for Western Blot, Immunoprecipitation, ELISA and supershift assays.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.01% sodium azide.
Specificity：Recognizes human NFkB p65 (RelA).
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Human NFkBp65 (Rel A) N-terminal peptide (KLH).
