Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism.
Lactic acid is produced as the end product of glycolysis.
Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions.
All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia.
Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis.
Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis.
Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells.
Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs).
At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.
MCT7/MOT7/SLC16A6 (mouse, 523-aa; human 423-aa, chromosome 17q25.1) is a new member of MCT family.
According to new nomenclature, the old MCT6 has now been reclassified as MCT7.
Source of Antigen and Antibodies:A 20-aa peptide sequence (designated MCT75-P; control peptide) within the Cytoplasmic, C-terminus of human MCT7 (1) was synthesized, coupled to KLH, polyclonal antibodies generated in rabbits.
Antibodies have been affinity purified over the control-peptide Sepharose.(Click here for a general protocol).
Applications: Western blot: 1–5ug/ml using ECL.
Immunohistochemistry: not tested.
It is recommended that the antibody be tried at 5–10ug/ml on paraformaldehyde fixed tissue.
ELISA: 1:10,000–1:100,000 using 50–100ng control peptide per well.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Affinity Purified Ig
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human Monocarboxylate Transporter 7 (MCT7/MOT7). The immunogen shows no significant sequence homology with other MCT.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 20aa peptide sequence within the cytoplasmic, C-terminus of human MCT7 (1).
