Phosphoglucomutase (PGM) interconverts the 1- and 6-phosphate isomers of alpha-D-glucose.
Although it is a reversible reaction, the formation of glucose-6-phosphate is markedly favored.
PGM will not convert beta-D-glucose-1-phosphate or beta-D-glucose-6-phosphate.
Applications:Suitable for Western Blot and ELISA as well as other antibody based assays using streptavidin or avidin conjugates.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000 to 1:25,000.
Against 1ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using Peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at RT.
Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Grade：Highly Purified
Purity：Purified from monospecific antiserum by salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder from PBS, 10mg/ml BSA (IgG and Protease free), pH 7.2, 0.01% sodium azide. Labeled with Biotin. Reconstitution: Reconstitute with 100ul sterile dH2O.
Specificity：Recognizes the 34kD enzyme. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified b-Phosphoglucomutase.
