The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal.
GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted.
GFP technology has revealed considerable new insights into the physiological activities of living cells.
Applications:Suitable for use in ELISA, Electron Microscopy, Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence/Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000Western Blot: 1:1000-1:3000Immunofluorescence (IC): 1:10-1:500Immunohistochemistry: 1:10-1:500Immunohistochemistry (Paraffin): 1:10-1:500Immunoprecipitation: 1:10-1:500Optimal dilution to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Green Fluorescent Protein (GFP). Species crossreactivity: all variants of Aequorea victoria GFP; S65T-GFP, RS-GFP, YFP and EGFP.
Isotype：IgG
Calc Applications Abbrev：E FC IC IF IHC IP WB
Immunogen：Highly purified recombinant (full length) GFP made in E. coli.
