Pro-inflammatory cytokines activate the transcription factor NF-kappa B by stimulating the activity of a protein kinase that phosphorylates Ikappa B, an inhibitor of NF-kappa B, at sites that trigger its ubiquitination and degradation.
A large, cytokine-responsive Ikappa B kinase (IKK) complex has been purified and the genes encoding 2 of its subunits have been cloned.
These subunits, IKK-alpha and Ikk-beta, are protein kinases whose function is needed for NF-kappa B activation by pro-inflammatory stimuli.
IKK is composed of similar amounts of IKK-alpha, Ikk-beta, which are differentially processed forms of a third subunit, IKK-gamma.
Ikk-gamma interacts preferentially with IKK-beta and is required for the activation of the IKK complex.
For Western Blotting, use at a dilution of 1:500 to 1:1000 (ECL).
However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified IgG
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human IKK gamma, NT. Other species not tested.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：N-terminus of the IKK-gamma protein, residues 2-13 (NRHLWKSQLCEM)
