84-1038-52　［取扱停止］Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro) 100ul　C1036-02A

特徴

• Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER).
• It is also a molecular chaperone and is involved in protein folding events in the ER.
• Calreticulin has also been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression.
• Calreticulin may play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (2-3).
• Recently it has been shown that calreticulin is induced in response to various types of cell stress including amino acid deprivation (1).
• Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years.
• Calreticulin might be centrally located and crucially participate in the coordination of these functions by the cell (2-3).
• Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1,000 (ECL).
• A band is detected at ~55kD
• Immunocytochemistry: 1:250Immunohistochemistry: 1:50Immunofluorescence: 1:50Immunoprecipitation: 1:300Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.