84-1038-47　ABCA1 (ABC 1, ABC Transporter 1, ATP-binding Cassette 1 antibody, anti-ATP-binding Cassette Transporter 1, CERP, Cholesterol Efflux Regulatory Protein, HDLDT1, TGD) 100ul　A0004

特徴

• Mutations in the ABC A1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD).
• TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking.
• ABC A1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell.
• It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux.
• Regulation of the cholesterol flux between HDL and macrophages is competitive between ABC A1 and SR-BI.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation Immunoflourescence, Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:250.
• Band representing ABC A1 is seen at ~220kD.
• Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC A1 signal
• Note: It is important not to boil the sample before loading onto a gel.
• Boiling can cause aggregation in large proteins, resulting in the protein’s inability to enter the gel.
• Post-nuclear lysates of 9-cis-retinoic acid and 22R-hydroxycholesterol induced raw macrophages were used for the positive control experiments
• Immunoprecipitation: See Krimbou and/or Denis, et al.
• references
• Immunohistochemistry: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.