Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H.
NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa.
Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture.
NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease or Alzheimerís disease.
Applications:Suitable for use in Immunohistochemistry, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot 1:5000 Immunohistochemistry 1:1000.
Cryostat sections and formalin-fixed paraffin-embedded sectionsImmunofluorescence (1:1,000)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220kD). Species crossreactivity: Human, feline, rat, bovine, mouse and porcine.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Bo Fe Hu Mo Po Rt
Immunogen：Recombinant C-terminal extension of rat NF-H
