84-1038-21 ARF, p19 100ul A3330-09A
特徴
- In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF.
- Although both act as cell proliferation inhibitors, their mechanisms of action are different.
- p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals.
- Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors.
- This result indicates that p19ARF is an important tumor suppressor.
- In addition, p19ARF helps prevent transforming signals from various oncoproteins via the p53 regulatory loop (3).
- These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.
- By Western blot, this antibody recognizes IVT murine p19ARF and a clear band of the same size in CTLL2 cells.
- It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs (1).
- The antibody can be competed from recognizing p19ARF using the antigenic peptide at 10ug/ml.
- Recommended Dilution:Western Blot: 0.5-1ug/ml (ECL)Immunohistochemistry:Not determinedELISA (indirect): 1:5,000
仕様
- Size：100ul
- Host：rabbit
- Source Antibody：mouse
- Grade：Affinity Purified
- Purity：Purified by affinity chromatography.
- Form：Antisera was eluted with 100 mM glycine, pH 2.5, and dialyzed against PBS, pH 7.4, 0.02% sodium azide.
- Specificity：Specific for p19ARF. Species crossreactivity: Reacts with mouse p19ARF. Does not react with rat and human.
- Isotype：IgG
- Calc Applications Abbrev：E WB
- Calc Crossreactivity：Mo
- Immunogen：KLH coupled peptide containing amino acids 54-75 of mouse p19ARF.
- Shelf Life：1year
- EU Commodity Code：30021010
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