Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D.
Each of these homolgs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes.
XRCC3 (x-ray cross complementing) was first identified as a human gene complementing the DNA damage sensitivity, chromosonal instability and impaired growth of the mutant hamster cell line irs1SF.
This fact coupled with XRCC3 being a member of the RAD51 gene family have implied a role in the repair of DNA damage by homologous recombination.
By Western blot, this antibody recognizes a band at 38kDa, representing XRCC3.
There are multiple bands for TK6 and HeLa cell extracts (this is normal and seen with XRCC3 recombinant protein).
The band just above the XRCC3 band does not appear in nuclear extracts.
Suggested Working Diultions:Western Blot-1:5,000 (ECL, using 100 ug Hela lysate)
Positive control: TK6 lysates and HeLa lysates, whole cell)
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Specific for XRCC3. No crossreactivity was observed with XRCC2, RAD51, RAD51B, RAD51C or RAD51D. Species crossreactivity: Reacts with human XRCC3.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic partial peptide of human XRCC3.
