84-1038-17　［取扱停止］Mre11 [Knockout Validated] 50ul　M4687

特徴

• The hMre11-hRad50-NBS1 protein plays a central role in the human cellular DNA Damage response, with recent studies indicating that these proteins help link DNA-damage detection to DNA-repair and cell cycle-checkpoint functions.
• This protein complex has been implicated in the activation of cell cycle-regulatory pathways, due to the fact that the NBS1 gene mutates in the chromosomal-instability syndrome, Nijmegen breakdown syndrome (NBS), which is characterized by increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity.
• HMre11-hRad50 foci form in response to DNA double-strand breaks and rely upon a DNA damage-induced signaling pathway.
• However, hMre11 migrates to sites of damage while hRad51 does not localize at these sites.
• These findings are consistent with the distinct role of these proteins in DNA repair.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence.
• Knockout Validated.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:10,000 in human and mouse Immunofluorescence: 1:200 in human (has not been tested in mouse) Immunoprecipitation: 3ul for 3x10e6 cells (in human and mouse) Optimal dilution determined by the researcher.
• Positive Control:Hela whole cell lysate.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.