84-1038-15　Hypoxia Inducible Factor 1a (HIF-1a) 100ul　H9807-04

特徴

• Hypoxia Inducible Factor 1 (HIF-1) is a nuclear protein involved in mammalian oxygen homeostasis.
• This occurs as a posttranslational modification by prolyl hydroxylation.
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits.
• Both subunits are constantly translated.
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes.
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity.
• Therefore, the concentration of HIF-1 alpha increases in the cell.
• Applications:Suitable for use in Western Blot, Gel Shift Assays, Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot:1:500-1:1000Immunoprecipitation: 1:1000Chromatin Immunoprecipitation: 25ul for 15 million cells
• Nuclear extracts are recommended.
• Immunohistochemistry (Frozen): 1:100-1:500Immunohistochemistry (Paraffin): 1:100-1:300 Requires HIER using sodium citrate buffer pH6.0 Mixed results with mouse
• Gel Shift Assay: 1:1-1:100 Immunofluorescence (IC): 1:100-1:500Optimal dilutions to be determined by the researcher.
• Positive Controls:RCC4 lysates, COS-7 hypoxic induced control lysate, PC12 nuclear extracts.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.