Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
HIF-1, a nuclear protein activating gene transcription in response to reduced cellular 02 concentration, activates the transcription of VEGF, EPO, iNOS, heme oxygenase 1 and other intracellular responses critical to hypoxia.
HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits.
HIF-1 a most closely resembles the Drosophila protein Sim.
HIF-1 b is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products.
(1,5,6)
Cellular Localization:HIF-1 beta is a nuclear protein.
Positive Control:MCF7 human nuclear extracts
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as serum, 0.02% sodium azide
Specificity：Specific for HIF-1 beta/ARNT. Crossreactivity with with ARNT2, which is related to HIF-1 beta/ARNT but is the product of a different gene, is not known. Species crossreactivity: Human, bovine, sheep, mouse, rat and ferret HIF-1 beta.
