ATM (ataxia telangiectasia mutated kinase), the master regulator of DNA double-strand breaks (DSBs) repair pathway, is a serine/threonine protein kinase that act as DNA damage sensor by activating checkpoint signaling upon DSBs, apoptosis and genotoxic stresses.
ATM activation involves its recruitment to DSBs through interaction with the MRE11-RAD50-NBS1 or MRN complex, followed by KAT5/TIP60 mediated acetylation.
ATM recognizes the substrate consensus sequence [ST]-Q and phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at DSBs, thereby regulating DNA damage response mechanism.
ATM also implicates in vesicle and/or protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, signal transduction, cell cycle control and act as a tumor suppressor.
ATM exist as dimers or tetramers in inactive state and on DNA damage, autophosphorylation dissociates ATM into monomers rendering them catalytically active and binds/activates ABL1, SAPK, DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C.
ATM is an integral part of BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex) and interacts with DCLRE1C, KAT8, KAT5, NABP2, ATMIN, CEP164, AP2B1, AP3B2, TELO2, TTI1, DDX1 etc.
NUAK1/ARK5 mediated ATM phosphorylation and ATM autophosphorylation at Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-regulated activation of the kinase.
Defects in ATM are the cause of ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL), B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL), B-cell chronic lymphocytic leukemia (BCLL).
Applications:Suitable for use in Immunofluorescence/Immunocytochemistry,Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000, detects a band at ~350kDImmunocytochemistry/Immunofluorescence: 1:100Immunohistochemistry (Paraffin): 1:100Immunoprecipitation: 1:10-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human ATM. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E FC IC IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence).
