84-1038-07　［取扱停止］APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1) 100ul　A2298-74A

特徴

• The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA.
• In addition, this enzyme has been shown to function as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1a as well as other transcription factors.
• Recently, APE has also been shown to control p53 activity through redox alteration.
• APE is also linked to apoptosis, associated with thioredoxin, and altered levels of APE have been found in some cancers (3).
• APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control (4).
• Applications:Suitable for use in Western Blot, Simple Western, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Blocking/Neutralization and Immunofluorescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 (ECL).
• Detects a single band at 37kD.
• Simple Western: 1:12.5Immunohistochemistry (Frozen/Paraffin): 1:100Immunofluorescence (IC): 1:50-1:200Immunoprecipitation: 7ug/mlChromatin Immunoprecipitation (ChIP): 1:10-1:500Blocking/Neutralization: 50-200 molar excessOptimal dilutions to be determined by researcher.
• Positive Control: HeLa whole cell extract
• Cellular Localization:In immunohistochemical experiments punctate nuclear staining is seen in most cell types.
• However, in hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.