Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system.
It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium.
Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors.
Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, mol wt.
~130-160kD).
One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA).
It is found in endothelium, adrenal glands, brain and platelets.
The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA.
It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.
Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).
Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible.
Sequence homology among different cloned isoforms is ~ 50%.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:ELISA: Control peptide should be coated at 1ug/ml
Western Blotting: 1:100-1:1000.
Recognizes 130kD protein
Immunohistochemistry: Not recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Control peptide: Catalog# I0800-09A
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid with 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II). Sequence is 84% conserved in human and rat (16/19 aa;). No significant sequence homology is seen with NOS-1/NOS-3 or other proteins. Antibody crossreactivity in various species is not established.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：C-terminal 19 amino acid peptide from mouse macrophage nitric oxide synthase. Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins
