Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system.
It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium.
Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors.
Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD).
One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA).
It is found in endothelium, adrenal glands, brain and platelets.
The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA.
It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.
Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).
Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible.
Sequence homology among different cloned isoforms is ~ 50%.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000.
Recognizes 130kD protein.
Optimal dilutions to be determined by the researcher.
Control Peptide:I0800-09A
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II). No significant sequence homology of immunizing peptide is seen with NOS-1/NOS-3 or other proteins. Human and rat peptides are 85% homologous with mouse peptide (12/14aa).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide mapping to 14 amino acids within the C-terminal of mouse nitric oxide synthase (KLH coupled). Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins.
