Applications: Suitable for use in Western Blot in Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2.0ug/ml detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells.
HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection systemImmunoprecipitation: 4ug immunoprecipitates p70 S6 kinase from 500ug of HEK293 RIPA lysate
Optimal dilution determined by the researcher.
Immunoprecipitation Kinase Assay: 5ug immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1uM microcystin.
The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes p70 S6 kinase Mr ~64kD. In some cell lysates, an additional protein may be detected at a higher molecular weight. Crossreacts with rat, human and mouse.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues 26–43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.
