VR1 (vanilloid receptor 1) is an ion channel activated by capsaicin, the pungent ingredient in hot peppers, and by noxious thermal heat.
It is expressed by and isolated from nociceptive sensory neurons.
Sequence analysis suggests that VR1 has transmembrane domains and intracellular N- and C-termini.
It shares structural similarities with the family of putative store-operated calcium channels.
It is thought to function as a transducer of noxious thermal stimuli and have involvement in inflammatory pain.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000--5000 using ECL.
Recombinant VR1 is ~90kDELISA: 1:10,000--50,000.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:C1078-01E: Capsaicin Receptor, Rat, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes rat Capsaicin Receptor. No significant sequence homology with VRL-1 or TRP or other proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 15aa to the C-terminus, cytoplasmic domain of rat Capsaicin Receptor. Species Sequence Homology: guinea pig, mouse and chicken; 93%, human; 63%
