SOX9 is a member of the family of SOX (Sry-type high mobility group box) gene that were first identified on the basis of region with high homology to that of Sry (Sex-determining region Y).
It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that closely parallels that of the gene for type II collagen.
Phosphorylation of SOX9 by PKA increases its DNA binding and transcriptional activity.
SOX9 regulates expression of chondrocyte phenotype related genes.
IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX9 mRNA and/or protein in chondrocytes, thus strongly inhibiting the expression of genes for cartilage extracellular matrix proteins.
The down-regulation of Sox9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.
Applications:Suitable for use in ELISA, Dot Blot, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Dot Blot: 1ug/mlWestern Blot: 0.5-2ug/ml; Detects Sox9 in mouse embryo cell lysate (Band at ~80kD)
Immunohistochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide.
Specificity：Recognizes human Sox-9 (~65kD). Species Crossreactivity: mouse, rat, chicken and frog
Isotype：IgG
Calc Applications Abbrev：DB E IHC WB
Calc Crossreactivity：Bo Ch Fr Hu Mo Rt
Immunogen：Synthetic peptide surrounding Serine 181, RRKSVKN of human Sox 9.
