84-1037-71　ZAP 70, phosphorylated (Tyr493) (TCR Zeta Associated Protein kinase 70kD) 100ul　Z0300-02

特徴

• ZAP70, a Syk family protein tyrosine kinase expressed in T and NK cells, plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1).
• Following TCR engagement, ZAP70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck (2-6).
• Tyrosine phosphorylation of ZAP70 correlates with its increased kinase activity and couples to downstream signaling events.
• Phosphorylation of Tyr493 within the activation loop results in enzymatic activation of ZAP70 (7).
• Phosphorylation of ZAP70 is crucial to the recruitment of additional molecules, such as Vav, Ras, and PLC gamma-1, to the membrane-associated signaling complex and for the induction of downstream signaling events.
• Mutation of the ZAP70 genes results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans.
• Recent evidence has suggested that ZAP70 should be considered as an effective biomarker in Chronic Lymphocytic Leukemia (CLL).
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.