The human X-chromosome-linked inhibitor of apoptosis protein XIAP is a major member of the inhibitor of apoptosis (IAP) family (1).
XIAP functions by direct binding and inhibition of several caspases, including caspase-3, caspase-7 and caspase-9 (2,3).
There are three BIR (baculovirus IAP repeat) domains in XIAP: BIR1, BIR2 and BIR3.
BIR2 has been shown to be important for the binding and inhibition of caspase-3 and caspase-7 (4,5), and BIR3 is a specific inhibitor of caspase-9 (5).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous levels of total human XIAP protein. Does not crossreact with other inhibitors of apoptosis. Species Crossreactivity: mouse, monkey and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the N-terminal of human XIAP.
