Tyrosine hydroxylase catalyzes the rate limiting step in the synthesis of dopamine and other catecholamines.
The enzyme’s activity is regulated at the levels of transcription, translation and post-translational modification.
Tyrosine hydroxylase exists in several different isoforms ranging from 50kD to about 62kD in human.
The N-terminal regulatory domain contains three phospho-serine sites: Ser19, Ser31 and Ser40.
Phosphorylation at Ser40 by PKA positively regulates the enzyme catalytic activity of the enzyme.
Applications:Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunofluorescence (IF-IC): 1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of rat tyrosine hydroxylase only when phosphorylated at serine 40.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Rt
Immunogen：Synthetic phosphopeptide corresponding to the sequence surrounding Ser40 of human tyrosine hydroxylase. Species sequence homology (100%): human and mouse.
