Some important signaling proteins can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position.
For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the-3,-2 and +2 positions (1–2).
c-Raf, a mitogen-activated protein kinase and the main effector recruited by GTP-bound Ras, is phosphorylated at Thr481 and Thr491 followed by Lys at the +2 position (3).
APP1 and ERF also contain a phosphorylated Thr followed by Arg or Lys at the +2 position.
Phosphorylation of these sites is important for their activities.
In order to determine the phosphorylation state of Thr in the Thr-X-Arg motif and to identify potential new phosphorylation sites in this motif, antibodies have been developed that recognize phosphorylated Thr followed by Arg or Lys at the +2 position.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:800 Requires HIER using sodium citrate buffer pH6.0 prior to staining
ELISA Peptide: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of proteins containing the phospho-Thr-X-Arg motif. Detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for the Lys compared to Arg at the +2 position. Does not crossreact with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. Recognizes all species. Species Crossreactivity: human, mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide containing the phospho-Thr-X-Arg motif.
