Syk is one of the well known protein tyrosine kinases.
It is widely expressed and plays an important role in intracellular signal transduction in hematopoietic cells (1-3).
Syk has been shown to interact with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4).
It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses including proliferation, differentiation and phagocytosis (4).
There is also evidence of a role for Syk in non-immune cells.
Syk may be a potential tumor suppressor in human breast carcinomas (5).
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous levels of total human Syk at 72kD. Detects both phosphorylated and nonphosphorylated forms of Syk. Does not crossreact with other members of the Syk/Zap-70 tyrosine kinase family. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the C-terminal sequence of human Syk (KLH).
