Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression.
Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4).
In addition, Stat6 is activated by IFNa in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6).
Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Immunoprecipitation: 1:100Immunofluorescence (IF-IC): 1:100 requires methanol permeabilization Flow Cytometry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human Stat6 only when phosphorylated at Tyr641 (MW 110kD). Does not crossreact with the corresponding phospho-tyrosine residues of other Stat proteins.
Isotype：IgG
Calc Applications Abbrev：FC IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues around Tyr641 of human Stat6. Species Sequence Homology: bovine 100%
